Chii chinonzi Sparger muFermenter?
Muchidimbu, ASparger mune FermenterChishandiso Chinoshandiswa Kusuma Mhepo kana Mamwe Magesi Mumudziyo Wekuvirisa.
Iyo inowanzoita pombi ine perforated iri pazasi pemudziyo kana padyo nemhepo uye inobvumira gasi kuburitswa mumvura kuburikidza nemakomba madiki kana jets.
Chinangwache sparger kupa oxygen kune aerobic fermentation process kana kusanganisa carbon dioxide (co2) yeanaerobic fermentation process. iyo sparger inobatsira kuchengetedza yakaringana mwero weokisijeni yakanyungudika mutsika yepakati, iyo yakakosha pakukura uye metabolism yezvipembenene, senge mbiriso kana mabhakitiriya.
Munguva yekuvirisa, sparger inogona zvakare kushandiswa kudzora iyo PH, tembiricha uye kusanganiswa kwemukati memudziyo. inowanzoshandiswa muindasitiri-scale bioprocessing, uko mavhoriyamu makuru ehutachiona ari kugadzirwa panguva imwe chete, kuve nechokwadi chekuvirisa kunoenderana uye kunoshanda.
Mumunda wekuvirisa, spargers inoita basa rakakosha mukugadzira nzvimbo yakakwana yekukura kwehutachiona. Nemashoko akapfava, sparger mudziyo unoshandiswa kupinza gasi (kazhinji oxygen kana kabhoni dhaokisaidhi) mumhepo inoyerera. Munguva yekuvirisa, spargers inowanzoshandiswa kudzora huwandu hweokisijeni yakanyungudika, chikafu chakakosha chekukura kwehutachiona. Mune ino blog post, isu tichakurukura zvakadzama kuti chii chinonzi spreader, kuti chinoshanda sei, uye kukosha kwayo mukuvirisa.
Chii chinonzi jekiseni?
Sparger chinhu chinoshandiswa kupinza gasi (kazhinji oxygen kana carbon dioxide) mumhepo inoyerera. Vashambadziri vanowanzo sanganisira yekupinza gasi uye chinhu chine porous kuparadzira gasi mumvura. Porous zvinhu zvinogona kugadzirwa kubva kwakasiyana zvinhu zvinosanganisira sintered simbi, ceramics kana mapolymers.
Spargers inowanzoshandiswa kudzora huwandu hweokisijeni yakanyungudika mune yemvura media panguva yekuvirisa. Izvi zvinodaro nekuti okisijeni ndicho chikafu chakakosha chekukura kwehutachiona uye kuwanikwa kwayo kunokanganisa zvakanyanya kumhanya uye kugona kwekuvirisa. Pamusoro pezvo, vadzidzisi vanogona zvakare kushandiswa kudzora pH yesvikiro yekuvirisa nekuunza kabhoni dhaikisaidhi kana mamwe magasi ane acidic.
Rudzi rwemugovera:
Kune akati wandei marudzi evaparadziri anowanzoshandiswa muindasitiri yekuvirisa, imwe neimwe iine zvayakanakira nezvayakaipira. Mhando dzinonyanya kupararira ndeidzi:
1. Porous Distributors:
Aya anogovera anogadzirwa nezvinhu zvine porous senge sintered simbi, ceramics kana polymers. Ivo vanowanzoshandiswa muindasitiri nekuda kwehukuru hwavo hwepamusoro uye nyore kushandisa.
2. Bubble jekiseni:
Majekiseni aya anopinza gasi munzvimbo yekuvirisa nenzira yemabhuru. Izvo hazvina kunyatsoshanda kupfuura porous spargers, asi inowanzoshandiswa pakuvirisa zviduku.
3. Membrane spargers:
Aya ma spargers anoshandisa gasi permeable membrane kupinza gasi mukati mekuvirisa svikiro. Izvo zvinoshanda, asi zvakaoma kuchenesa nekuchengetedza.
Kukosha kwemaspager mumavirira:
Spargers inoita basa rakakosha mukuvirisa nekudzora iyo yakanyungudika okisijeni kusangana uye pH mune yekuvirisa svikiro. Iko kusanganiswa kweokisijeni yakanyungudika ndiyo yakakosha parameter yekukura kwehutachiona sezvo ichikanganisa mwero uye kugona kwekuvirisa. Nekudzora kuwanda kweokisijeni yakanyungudika, spargers inogona kubatsira kuve nechokwadi chekuti nzira yekuvirisa yakagadziridzwa kuitira goho repamusoro uye mhando.
Pamusoro pekudzora kuwanda kweokisijeni yakanyungudika, nebulizers inogona zvakare kushandiswa kudzora iyo pH yekuvirisa svikiro. Izvi zvakakosha nekuti pH inokanganisa zvakanyanya chiyero uye kugona kwekukura kwehutachiona. Nekuunza maasidhi egasi senge kabhoni dhaibhorosi, spargers inogona kubatsira kuchengetedza yakakwana pH renji yekukura kwehutachiona.
Zvinhu zvekufunga nezvazvo pakusarudza spreader:
Pakusarudza sparger yekuvirisa, zvinhu zvakati wandei zvinoda kutariswa, zvinosanganisira rudzi rwetwupukanana turi kugadzirwa, ukuru hwechinovirisa, uye kuvirisa kwaunoda. Semuyenzaniso, maporous spargers anowanzo fanira kuvirisa mahombe nekuda kwekushanda kwawo kwepamusoro, nepo air bubble spagers angave akanyatsokodzera kuvirisa kwezvidiki-diki. Sarudzo yesparger inogona zvakare kuenderana nezvinodiwa chaizvo zvekuvirisa maitiro, senge inodiwa yakanyungudika oksijeni concentration kana iyo optimum pH renji.
Kuchengetedza uye Kucheneswa kweSpreader:
Kugadzirisa kwakaringana sparger uye kuchenesa kwakakosha kuti uve nechokwadi chekuita kwakanyanya uye kudzivirira kusvibiswa. Spargers dzinofanirwa kucheneswa nguva nenguva kuti dzibvise chero kuvaka kwekukura kwehutachiona kana zvimwe zvinosvibisa zvinogona kukanganisa kugona kwavo. Nzira yekuchenesa inogona kusiyana zvichienderana nemhando ye sparger, asi inowanzobatanidza kunyura sparger mumushonga wekuchenesa, uchiteverwa nekunyatsosuka nemvura. Pamusoro pekuchenesa nguva dzose, sparger inofanira kuongororwa nguva dzose kuitira kukanganisa kana kusakara uye kuchinjwa sezvinodiwa.
Mhando dzespargers dzinoshandiswa mukuvirisa
1. Porous Distributor:
Porous spargers inogadzirwa kubva kwakasiyana-siyana zvinhu zvinosanganisira sintered simbi, ceramics uye polymers. Aya ma spargers ane nzvimbo yakakwirira yepamusoro uye nhamba huru yediki pores, iyo inobvumira gasi kuti rigovane zvakaenzana mukati mekuvirisa kwepakati. Kushanda kwepamusoro kweporous spagers kunoita kuti ive sarudzo yakakurumbira yekuvirisa kukuru, uko kukwirisa gasi kutakurisa mitengo kwakakosha pakukura kwakakwana kwehutachiona. Porous spargers zvakare ine mukana wekuve nyore kuchenesa nekuchengetedza, sezvo inowanzogadzirirwa kuparadzaniswa uye kucheneswa pakati pemabhechi.
2. Bubble Shooter:
Bubble majekiseni, anozivikanwawo semadiffuser, anounza gasi mukati mekuvirisa svikiro nenzira yemabhuru. Aya ma spargers anowanzo gadzirwa neine porous material kana fine mesh inogadzira tuturu twemhepo kana gasi richipfuura. Majekiseni eBubble anowanzo shandiswa pakuvirisa zvidiki-diki uko mazinga ekufambisa gasi akanyanya kukosha. Mabubble spargers ane mukana wekuve nyore uye asingadhure kushandisa, asi anogona kunge asina kunyatso shanda pane ane porous spargers uye anokonzeresa bongozozo munzvimbo yekuvirisa.
3. Membrane distributor:
Membrane spargers anoshandisa gasi permeable membranes kupinza gasi mukati mekuvirisa. Aya ma spargers ane nzvimbo yakakwirira yepamusoro uye nhamba huru yediki pores, iyo inobvumira gasi kuti rigovane zvakaenzana mukati mekuvirisa kwepakati. Membrane spargers inoshanda uye inogona kutamisa gasi nekumhanya kwakanyanya, zvichiita kuti ive sarudzo yakakurumbira yekuvirisa kwakakura. Membrane spargers, zvisinei, inogona kunyanya kuoma kuchenesa nekuchengetedza nekuti membrane inowanzotetema uye kukuvara nyore.
4. Surface Aerators:
Surface aerators, inozivikanwawo semagitators, inogadzira kuyerera kwemhepo pamusoro peiyo yekuvirisa svikiro, zvichiita kuti gasi ripinde mumvura. Aya madhivhari anowanzo shandiswa mudiki kuvirisa uko miyero yekufambiswa kwegasi yakanyanya kusanyanya kukosha. Maeerators epamusoro ane mukana wekuve nyore uye asingadhure kushandisa, asi anogona kunge asina kunyatso shanda pane mamwe marudzi esparger uye kugadzira mhirizhonga yakawanda mumhepo yekuvirisa.
5. Sirinji:
MaEductors anoshandisa high-pressure gasi jets kugadzira nyonganyonga mumhepo yekuvirisa, zvichiita kuti gasi ripinde mumvura. Aya anoparadzira anowanzo shandiswa mudiki kuvirisa uye ane mukana wekuve nyore kushandisa uye isingadhure. Zvakadaro, majekiseni anogona kusashanda zvakanyanya pane mamwe marudzi espager uye kugadzira mhirizhonga yakawanda mumhepo yekuvirisa.
Sarudza chiparadzi chakakodzera:
Pakusarudza sparger yekuvirisa, zvinhu zvakati wandei zvinoda kutariswa, zvakaita serudzi rwetwupukanana twuri kugadzirwa, ukuru hwechinovirisa, uye kuvirisa kwaunoda. Sarudzo yesparger inogona zvakare kuenderana nezvinodiwa chaizvo zvekuvirisa maitiro, senge inodiwa yakanyungudika oksijeni concentration kana iyo optimum pH renji. Kugadzirisa kwakaringana sparger uye kuchenesa kwakakosha kuti uve nechokwadi chekuita kwakanyanya uye kudzivirira kusvibiswa. Nekunzwisisa mhando dzakasiyana dzevaparadziri varipo uye zvakanakira nezvazvakaipira, nyanzvi dzekuvirisa dzinogona kuita sarudzo dzine ruzivo nezve nzira yekukwirisa maitiro ekuvirisa uye kuwana mhedzisiro yaunoda.
Basa guru re sparger mu fermenter
1. Oxygenation:
Rimwe remabasa makuru esparger mune yekuvirisa ndeyekupa oxygen kune tupukanana turi kugadzirwa. Izvi zvinonyanya kukosha kune aerobic microorganisms inoda oxygen yekukura uye metabolism. Iyo sparger inoendesa okisijeni kunzvimbo yekuvirisa nenzira yemabhuru emhepo kana kuyerera kwemhepo, zvichiita kuti hutachiona hutore mweya weokisijeni uye woishandisa kugadzira simba.
2. Mix:
Pamusoro pekupa oxygen, sparger inoitawo basa rakakosha mukusanganisa svikiro rekuvirisa. Kana gasi rinounzwa mukati, rinogadzira mhirizhonga, iyo inobatsira kugovera chikafu uye zvimwe zvakakosha zvinongedzo zvakaenzana mukati memvura. Kusanganisa kwakaringana kwakakosha kuti uve nechokwadi chekukura kwakakwana kwehutachiona sezvo ichibvumira tupukanana kuwana chikafu chavanoda uye kubvisa tsvina.
3. PH Kudzora:
Spargers inogonawo kushandiswa kudzora pH yekuvirisa svikiro nekuunza carbon dioxide kana mamwe magasi ane acidic mumvura. Izvi zvinobatsira kuchengetedza pH mukati meiyo yaunoda uye kudzivirira kukura kwehutachiona hunokuvadza hunobudirira munzvimbo dzine acidic.
4. Kudzora Tembiricha:
Mune zvimwe zviitiko, spargers inogona zvakare kushandiswa kudzora tembiricha yekuvirisa svikiro. Nekuunza kuyerera kwemhepo inotonhorera, sparger inogona kubatsira kuderedza tembiricha yenhau uye kudzivirira kupisa. Sezvineiwo, mhepo inopisa inogona kushandiswa kuwedzera tembiricha yepakati uye kukurudzira kukura kwehutachiona.
5. Foam Control:
Chekupedzisira, masprayers anogona zvakare kushandiswa kudzora kuumbwa kwefuro panguva yekuvirisa. Foam inogona kuva dambudziko mukuvirisa kukuru sezvo ichikanganisa kusanganisa uye inoderedza kugona kwemugovera. Nekuunza mafambiro emhepo munzvimbo yekuvirisa, spargers inogona kubatsira kuputsa furo uye kudzivirira furo kukwira.
mumhedziso:
Muchidimbu, spargers inoita basa rakakosha mukuvirisa nekupa oxygen, kusanganisa fermentation media, kudzora pH uye tembiricha, nekudzivirira kuumbwa kwefuro. Kusarudza iyo chaiyo mhando ye sparger uye kuichengeta nekuichenesa nemazvo kwakakosha kugonesa maitiro ekuvirisa uye kuwana mhedzisiro inodiwa. Nekunzwisisa mashandiro emugoveri mune yekuvirisa, nyanzvi dzekuvirisa dzinogona kuita sarudzo dzine ruzivo pamusoro pekugadzirisa maitiro ekuvirisa uye kuwana mhedzisiro inodiwa.
Kushandiswa kwe distributor mune fermenter
1. Kugadzirwa kwedoro newaini:
Imwe yeanonyanya kunyorera maparadziri mumavirisa ari mukugadzirwa kwedoro newaini. Mune maindasitiri aya, spargers inowanzoshandiswa kupa okisijeni kumbiriso panguva yekuvirisa, iyo inobatsira kukurudzira hutano hwembiriso kukura uye kuvirisa kunoshanda. Spargers inogona zvakare kushandiswa kubatsira kudzora tembiricha uye pH yekuvirisa svikiro, izvo zvakakosha kuti uwane inodiwa flavour uye doro nhanho muchigadzirwa chekupedzisira.
2. Bioremediation:
Spargers inoshandiswawo mumunda we bioremediation, iyo inosanganisira kushandiswa kwema microorganisms kuputsa zvinosvibisa uye zvimwe zvinosvibisa zvakatipoteredza. Muchishandiso ichi, spargers inoshandiswa kupinza oxygen munzvimbo yakasvibiswa, iyo inobatsira kusimudzira kukura kwehutachiona hunokonzera kuputsa zvinosvibisa. Iyi nzira inonyanya kushanda pakuchenesa ivhu nemvura yepasi yakasvibiswa nemafuta epetroleum, zvinonyungudutsa uye mamwe makemikari emaindasitiri.
3. Kugadzirwa kwemishonga:
Vashambadziri vanoshandiswawo zvakanyanya muindasitiri yemishonga yekugadzira majekiseni, mishonga inorwisa mabhakitiriya nemimwe mishonga. Mumashandisirwo aya, spargers inoshandiswa kuunza okisijeni mutsika dzehutachiona hunoita basa rekugadzira chigadzirwa chaunoda. Spargers inogonawo kubatsira kusanganisa iyo yekuvirisa svikiro uye kudzora tembiricha uye pH yetsika, izvo zvakakosha kuchengetedza hutano uye chibereko chetsika dzehutachiona.
4. Kuviriswa kwemaindasitiri:
Spargers zvakare inowanzoshandiswa mukuvirisa maindasitiri makuru kugadzira ma enzymes, amino acids, uye zvimwe zvigadzirwa zvebiological. Mumashandisirwo aya, spargers inoshandiswa kupa okisijeni kutsika yehutachiona uye kubatsira kuchengetedza tembiricha uye pH inodiwa netsika. Kukura kwakakodzera kwakakosha kuti uwane goho repamusoro uye kuchengetedza hutano uye kugadzirwa kwetsika dzehutachiona.
5. Tsvagiridzo nebudiriro:
Chekupedzisira, vaparadziri vanowanzo shandiswa mukutsvagisa nekusimudzira marongero kuti vadzidze kukura uye hunhu hwema microorganisms munzvimbo inodzorwa murabhoritari. Spargers inogona kushandiswa kuunza akasiyana magasi mune yekuvirisa midhiya, ichibvumira vaongorori kuti vadzidze mhedzisiro yezvakasiyana mamiriro ezvakatipoteredza pakukura kwehutachiona uye metabolism.
mumhedziso
Muchidimbu, spargers chikamu chakakosha chemaitiro mazhinji ekuvirisa, kupa oxygen, kusanganisa kuvirisa midhiya, uye kutonga tembiricha uye pH yetsika. Kushandiswa kwevashambadziri kwakakosha pakuwana goho repamusoro uye kuchengetedza hutano uye kugadzirwa kwetsika dzehutachiona. Nekunzwisisa mashandisirwo akasiyana siyana uye mashandisirwo ezviparadziso mukuvirisa, nyanzvi dzekuvirisa dzinogona kuita sarudzo dzine ruzivo nezve nzira yekukwirisa maitiro ekuvirisa uye kuwana mhedzisiro inodiwa.
Ndeipi inonzi Yakakurumbira Fermenter Sprayer?
nozzle sprayer mu fermenter uyeOrifice distributors mune fermenters
Nozzles uye orifice distributors imhando mbiri dzakakurumbira dzevashambadziri dzinoshandiswa mukuvirisa. Heano mamwe ruzivo pane imwe neimwe:
1. Nozzle distributors mune fermenters:
Nozzle sparger is sparger inoshandisa nhevedzano yemhino kupinza maturu emhepo mukati mekuvirisa. Manozzles anowanzo kurongeka mugrid pateni pazasi pefermenter iyo gasi inounzwa pamwero wakadzorwa. Nozzle spagers dzakakurumbira muindasitiri-chikero kuvirisa nekuti dzinopa kusanganisa kwakanaka uye oxygenation yekuvirisa svikiro. Iwo zvakare ari nyore kuchenesa nekuchengetedza.
2. Orifice distributors mune fermenters:
An orifice sparger (an orifice sparger) imhuka inonzi sparger inoshandisa tumaburi tudiki kana maburi kupinza mabubbles emhepo mukati mekuvirisa. Mamiriro acho anowanzo kurongedzerwa pateni yegridi muzasi peinovirisa iyo gasi inounzwa pamwero wakadzorwa. Orifice spargers yakakurumbira munzvimbo dzeR&D nekuti inobvumidza kunyatso kudzora magasi ekusuma magasi uye inogona kushandiswa kudzidza mhedzisiro yeakasiyana magasi ekuumbwa pakukura kwehutachiona uye metabolism.
Ose ari maviri nozzles uye orifice spargers anoshanda mukupa mweya weokisijeni kutsika yemicrobial uye kusanganisa iyo yekuvirisa svikiro. Kusarudzwa kwe sparger kunoenderana nezvinodiwa chaizvo pakuvirisa, kusanganisira ukuru hwechinhu chinovirisa, rudzi rwetwupukanana turi kugadzirwa, uye mhedzisiro inodiwa pakuvirisa. Pakupedzisira, chinangwa ndechekusarudza sparger inopa kufambisa gasi inoshanda uye inosimudzira hutano hwehutachiona kukura uku ichideredza kuumbwa kwefuro uye zvimwe zvisingadiwe mhedzisiro.
mukupedzisa:
Mukupfupisa, sparger chikamu chakakosha chekuvirisa uye inoita basa rakakosha mukudzora kuwanda kweokisijeni yakanyungudika uye pH mumhepo yekuvirisa. Kusarudza mhando chaiyo yekuparadzira nekuichengeta nekuichenesa nemazvo kunogona kubatsira kuve nechokwadi chekuvirisa kwakagadziridzwa kuitira goho repamusoro uye mhando. Nekunzwisisa kuti chiparadziri chii uye kuti chinoshanda sei, nyanzvi dzekuvirisa dzinogona kuita sarudzo dzine ruzivo nezve maitiro ekugadzirisa maitiro ekuvirisa uye kuwana mhedzisiro inodiwa.
Iwe unofarira kukwirisa maitiro ako ekuvirisa nevashambadziri?
Kana zvirizvo, usazeze kuongorora dzakasiyana siyana dzekuparadzira sarudzo dziripo kuti uone kuti dzinogona sei kubatsira yako chaiyo application.
Bata nyanzvi yekuvirisa uye sarudza kana OEM chaiyo sintered sparger nhasi kuti udzidze zvakawanda uye tanga kuvandudza maitiro ako ekuvirisa!
Zvimwe zvekushandisa:
Kune vaverengi vanofarira kudzidza zvakawanda nezve vaparadziri mune zvekuvirisa, akati wandei zviwanikwa zviripo. Zvimwe zvinokurudzirwa zvekushandisa zvinosanganisira:
"Fermentation Microbiology uye Biotechnology" naEMT El-Mansi, CFL Amaral uye WWM Souza (2019)
"Mitemo yeBioprocess Engineering" naPauline M. Doran (2012)
"Handbook of Industrial Cell Culture: Mammalian, Microbial, uye Plant Cells" Mupepeti-mukuru-Victor G. Aunins (2010)
Nguva yekutumira: May-05-2023
